SUMMARY:

In recent years, studies with non-steroidal anti-inflammatory drugs (NSAID) have been found to be effective in behavioral disorders such as anxiety. It is also stated that these drugs, which are used for the treatment of inflammation, fever, and pain, have neuroprotective effects in some studies. In particular, dipyrone is one of the most studied drugs for this purpose. These data indicate that this active substance might have an effect on neuronal activity and have the potential to provide a new area of use in this topic. With our proposed project, we plan to evaluate the effects of dipyrone on neuronal markers in the GT1-7 cell line. In addition, the results will be compared with indomethacin to reveal whether there is a general phenomenon for all NSAIDs in this regard. The study is based on the gene expression analysis of markers at the molecular level. The mRNA expression levels of nerve growth factor (NGF), beta-tubulin III and nestin will be determined in this research. In case of possible neuroprotective effects of dipyrone are gathered as a result of the study, the potential of using a low-cost active substance in neurodegenerative diseases will be revealed.